Table I.
Crosslinked ribosomal proteins identified by mass spectrometry
| Band | Protein Database Match | Predicted Molecular Mass | pI | GenBank Accession No. | Peptides Matched | Measured Mass | Computed Mass | Peptide Sequence |
|---|---|---|---|---|---|---|---|---|
| Species | kD | D | D | |||||
| 1 | 40S ribosomal protein S14 (Brassica napus) | 16.305 | 10.14 | AAO41731 | 2 | 1,053.53 | 1,053.56 | TPGPGAQSALR |
| 1,584.81 | 1,584.81 | IEDVTPIPSDSTRR | ||||||
| 2 | 60S ribosomal protein L32 (O. sativa) | 15.707 | 10.43 | BAC75414 | 1 | 1,510.88 | 1,510.88 | AAQLDIVVTNKLAR |
| 3 | 60S ribosomal protein L23a (Arabidopsis) | 17.462 | 10.20 | AAB87692 | 2 | 1,433.71 | 1,433.70 | LTPDYDALDVANK |
| 1,829.94 | 1,829.98 | LTPDYDALDVANKIGII | ||||||
| 4 | 50S ribosomal protein L29 (maize) | 18.082 | 10.85 | AAD50383 | 2 | 2,612.21 | 2,612.27 | AMTTEQMEEEVVDL KGELFLLR |
| 1,044.52 | 1,044.51 | EQELEEIR |
The highest statistically significant match for each protein is shown, with the determined molecular mass, calculated pI and GenBank accession numbers of these matches. Proteins were extracted from an SDS-PAGE gel and treated as described in “Materials and Methods” before mass spectrometry. Peptide masses obtained experimentally and calculated from composition are shown, along with the amino acid sequences deduced from patterns of fragmentation.