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. 2016 Jun 23;72(2):143–151. doi: 10.1093/gerona/glw093

Figure 1.

Figure 1.

Adaptation to H2O2 diminishes during aging and is dependent on SKN-1. (A) Dose survival curve for N2 animals exposed to H2O2. The LD50 dose was determined to be 6 and 7mM for Day 3 and Day 10 animals, respectively. (B) Oxidative stress tolerance for N2 animals adapted to 1- to 10-µM H2O2 preconditioning at Day 3 and Day 10 and challenged with LD50 determined in (A). (C) Survival assay for N2 animals exposed to 1-µM or 2-mM H2O2 for 20 minutes at Day 3. Significance determined by log-rank (Mantel-Cox) test. p = .2054, not significant. (D) Oxidative stress tolerance at 6mM for untreated versus preconditioned (1-µM H2O2) skn-1(zu67), hsf-1(sy441), hif-1 (ia4), and daf-16(m26) animals. Error bars denote standard error of the mean values. *p < .05, **p < .01, ***p < .001 relative to control using Student’s t test.