Figure 2.

SKN-1 recruitment to the pbs-5 promoter declines during aging. (A) Schematic representation of the α and β subunits of the 20S proteasome. (B) Representation of the pbs-5 putative promoter and the ARE-specific and nonspecific (NS) primers used for qPCR of SKN-1::GFP ChIP samples. This region is closely conserved for two other nematode species. (C) qPCR analysis of pbs-5 for Day 3 and Day 10 N2 animals in response to 2-mM H₂O₂. Expression levels were adjusted to N2 Day 3 control animals. (D) ChIP showing that SKN-1 is recruited to the pbs-5 promoter in response to H₂O₂ for Day 3, but not for Day 10 animals. (E) SKN-1 occupancy does not change in response to stress at a nonspecific site upstream of the pbs-5 promoter. Error bars denote standard error of the mean values. *p < .05, **p < .01, ***p < .001 relative to control using Student’s t test.