Figure 3.

Induction of phase II detoxification gene gst-4 declines in response to H₂O₂ in aged animals. (A) qPCR analysis of gst-4 for Day 3 and Day 10 N2 animals in response to 2-mM H₂O₂. Expression levels were adjusted to N2 Day 3 control animals for each gene. (B) SKN-1 is recruited to the gst-4 promoter in response to 2-mM H₂O₂ for Day 3, but not for Day 10 animals. (C) SKN-1 occupancy does not change in response to stress at a nonspecific site downstream of the gst-4 promoter. Error bars denote standard error of the mean values. *p < .05, **p < .01, ***p < .001 relative to control using Student’s t test. (D) Representation of the gst-4 putative promoter and the ARE-specific and nonspecific (NS) primers used for qPCR of SKN-1::GFP ChIP samples.