Figure 6.

Increased SKN-1 activation does not rescue adaptive proteolytic capacity during aging. (A) Animals with increased SKN-1 activity (lax120) have an increased tolerance for H₂O₂ compared with N2. The LD₅₀ dose was determined to be 30 and 50mM for Day 3 and Day 10 animals, respectively. (B) Oxidative stress tolerance for lax120 animals adapted to 1- to 10-μM H₂O₂ preconditioning at Day 3 and Day 10 and challenged with LD₅₀ determined in (A). (C) Increased activity of SKN-1 (lax120) does not rescue proteolytic capacity to degrade Suc-LLVY-AMC for Day 10 animals. (D) Increased activity of SKN-1 (lax120) does not rescue proteolytic capacity to degrade oxidized [3H] hemoglobin for Day 10 animals. Error bars denote standard error of the mean values. *p < .05, **p < .01, ***p < .001 relative to control using Student’s t test.