Table I.
Storage polysaccharide metabolism in plants and bacteria
| Genes in E. coli Genome | Genes in O. taurii Genome | Genes in Arabidopsis Genome | Genes in O. sativa Genome |
|---|---|---|---|
| AGPase | AGPase small subunit | AGPase small subunit | AGPase small subunit 1 and 2 |
| AGPase large subunit A and B | AGPase large subunit 1, 2, 3, and 4 | AGPase large subunit 1 and 2 | |
| α-Amylase A and B | α-Amylase A, B, and C | α-Amylase 1, 2, and 3 | α-Amylase 1 to 9 |
| 0 | β-Amylase A and B | β-Amylase 1 to 8 | β-Amylase 1 to 7 |
| GBE | SBE I, II-1, and II-2 | SBE, 2-1, and 2-2 | SBE 1 to 4 |
| 4-α-Glucanotransferase | 4-α-Glucanotransferase 1 and 2 | 4-α-Glucanotransferase 1 and 2 | 4-α-Glucanotransferase 1 and 2 |
| Glycogen debranching enzyme | Isoamylase A and B | Isoamylase 1, 2, and 3 | Isoamylase 1, 2, and 3 |
| Pullulanase | Pullulanase | Pullulanase | |
| GPHO and MPHO | PHO A, B, and C | PHO1 and PHO2 | PHO1 and PHO2 |
| 0 | R1 protein A and B | R1 protein | R1 protein |
| GS | GBSS I | GBSS I | GBSS I |
| SS I, II, IIIa, IIIb, and IIIC | SS I, II, III, IV, and V | SSI, Iia, IIb, III, III-2, IVa, and IVb | |
| 0 | 0 | Glycogenin 1 to 7 | Glycogenin 1 to 4 |
The protein-deduced from four different species was generated using Bioedit (Hall, 1999) and Forcon (Van de Peer et al., 1998). The species included were E. coli, O. tauri, Arabidopsis, and O. sativa. Amino acid sequence of O. tauri genome provided from the Ostreococcus blast research Web site (http://194.167.19.196/). Ostreococcus accession numbers for all reported sequences are given as supplemental material. Expression of all Ostreococcus sequences involved in starch metabolism was checked by RT-PCR and double checked by analysis of the Expression Sequence Tag Database. Amino acid sequences from other organisms were provided from the Arabidopsis Information Resource Web site (www.arabidopsis.org) and the RiceBlastDB Web site (http://www.tigr.org/tdb/e2k1/osa1/osa1.shtml; GenBank accession numbers are given as supplemental material). The following abbreviations were used: AGPase, ADP-Glc pyrophosphorylase; GS, glycogen synthase; SS, soluble starch synthase; GBE and SBE, respectively, glycogen and starch branching enzyme; GPHO and MPHO, glycogen and maltodextrin phosphorylase.