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. 2017 Jan 13;7:40797. doi: 10.1038/srep40797

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(A) Representative images of D1ER probe yellow fluorescence (YFP) and FRET ratio images of HeLa cells transfected with pcDNA3 (up) or TFEB (below), before and after stimulation with histamine (100 μM) and after washout of the perfused ER Ca²⁺-releasing stimulus. (B) Measurements of the Δ FRET ratio (ΔR/R_min) in HeLa cells co-transfected with pcDNA3 (control) or TFEB3xflag (TFEB) for 48 h during lysosomal emptying after histamine (100 μM) stimulation (n = 10 for each condition). (C) Ca²⁺ re-uptake rate measurements in pcDNA3 (control)- or TFEB3xflag (TFEB)-transfected cells during washout of the agonist (histamine 100 μM) (pcDNA3 n = 28, TFEB n = 20). Data are presented as the means ± SEM; ***p < 0.001.