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. 2017 Jan 13;7:40625. doi: 10.1038/srep40625

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Copyright © 2017, The Author(s)

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(A) Expression levels of MsHPPD in different organs of alfalfa. RNA was extracted from tissues collected from two-year-old alfalfa. Data presented are mean ± SD, each with three biological replicates and three technical replicates. Expression levels are relative to early flower; Statistical analyses were carried out via a two-tailed Student’s t-test, asterisks show the significance of P < 0.05. (B) Histochemical GUS staining of pHPPD::GUS lines. (a) Ten-day-old Arabidopsis seedling, bar: 1 cm; (b) Silique of transgenic Arabidopsis, bar: 200 μm; (c) Early flower, bar: 200 μm; (d) Late flower, bar: 200 μm; (e) Pollen, bar: 50 μm.