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. 2017 Jan 13;7:40625. doi: 10.1038/srep40625

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Copyright © 2017, The Author(s)

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(A) MsHPPD expression in three MsHPPD-overexpressing transgenic lines and wild-type, as measured by qRT-PCR. Total RNA was extracted from two-week-old MsHPPD-overexpressing transgenic Arabidopsis (OE2, OE6, and OE7) and control seedlings, before being subjected to qRT-PCR analysis. The expression levels of MsHPPD were normalized to At4g26410. Data are presented as mean ± SD, each with three biological replicates and three technical replicates. Statistical analyses were carried out via a two-tailed Student’s t-test with a significance of P < 0.05. (B) Histochemical GUS staining of MsHPPD-overexpressing lines (OE2, OE6, and OE7) and wild-type seedlings with pBI121 empty vector in the Col-0 background. Two-week-old transgenic and control Arabidopsis were used for GUS staining. Bar: 1 cm. (C) Expression analysis of endogenous genes involved in vitamin E biosynthesis in MsHPPD- overexpressing Arabidopsis (OE2, OE6, and OE7) and wild-type control. Two-week-old Arabidopsis seedlings were used for qRT-PCR. Data are shown as the mean ± SD with three biological replicates and three technical replicates. The expression levels of the genes detected in the experiment were normalized to At4g26410. Statistical analyses were carried out via a two-tailed Student’s t tests with a significance of P < 0.05.