Table 1. The primer sequences used in cloning and expression analysis.
Primers | Sequences | Application |
---|---|---|
3′RACE | GTGCCATTCCATCGTCTCCTCCA | RACE |
5′RACE | GGATTCTGGTTTGGGTTCGGGTC | RACE |
PBI-HPPD-F | CACGGGGGACTCTAGAATGGCCATCGAAACAGAAAC | Vector construction |
PBI-HPPD-R | AGGGACTGACCACCCGGGGATGCAGTTCTTCTAGTTTCCA | Vector construction |
AtHPPD-F | GCGGTTTAAATTCAGCGGTCCTG | Q-PCR |
AtHPPD-R | TCCGTGCACTGGCTCGTTAATC | Q-PCR |
AtHPT-F | TGGAGCAAAGTCATCTCGGTTG | Q-PCR |
AtHPT-R | GACTTAGCTCGAGCCCACAAAG | Q-PCR |
AtTC-F | TGTCCGAAGGGTTCCAAGCTAC | Q-PCR |
AtTC-R | CACAGTTTCCGCATAGTCAGTACG | Q-PCR |
AtMPBQMT-F | TGCCGGTTTCAAGGACGTTCAG | Q-PCR |
AtMPBQMT-R | TCTTCCTTTGGACCAAGCTGGAG | Q-PCR |
AtTMT-F | TGCTCAATCACTCGCTCATAAGGC | Q-PCR |
AtTMT-R | TCTTCGAATGGCTGATCCAACGC | Q-PCR |
VTE5-F | CAAGCGAAACGTCATTCAACAGAG | Q-PCR |
VTE5-R | GAAGATTGGCCACGCAAGTACG | Q-PCR |
AtHST-F | GCATGTTCTCAGGTTGGTGCTG | Q-PCR |
AtHST-R | AGCTCTTGTCACCAAGGCAGTG | Q-PCR |
AtABI3-F | GAAGCAAAGCGACGTGGGTAAC | Q-PCR |
AtABI3-R | AACCTGTAGCGCATGTTCCAAAC | Q-PCR |
AtABI5-F | AAACATGCATTGGCGGAGTTGG | Q-PCR |
AtABI5-R | CGGTTGTGCCCTTGACTTCAAAC | Q-PCR |
AtABA1-F | TGGGCTTGGTCCTCTGTCTTTC | Q-PCR |
AtABA1-R | CACCAACTCTTCCTGGATGTGG | Q-PCR |
AtRAB18-F | TCTAGCTCGGAGGATGATGGAC | Q-PCR |
AtRAB18-R | ACCGTAGCCACCAGCATCATATC | Q-PCR |
AtNCED3-F | TCGAAGCAGGGATGGTCAACAG | Q-PCR |
AtNCED3-R | GCTCGGCTAAAGCCAAGTAAGC | Q-PCR |
AtNCED5-F | CCTCCGTTAGTTTCACCAACACT | Q-PCR |
AtNCED5-R | GGTGTGTCGGAGACGGAGTT | Q-PCR |
AtNCED9-F | CCCAAACCGCAGCGTTTAAT | Q-PCR |
AtNCED9-R | GTTCGGTCGAGGAATTGGGT | Q-PCR |
AtABA3-F | TGCTACAAGGCTCCCCCTTT | Q-PCR |
AtABA3-R | CACAACCCTTTGCAGCATCA | Q-PCR |
AtAAO3-F | GAAGGTCTTGGAAACACGAAGAA | Q-PCR |
AtAAO3-R | GAAATACACATCCCTGGTGTACAAAAC | Q-PCR |
MsHPPD-F | CAGTTATCCTCGAAAACCACG | Q-PCR |
MsHPPD-R | GTTTGGGTTCGGGTCGTTG | Q-PCR |
Actin-F | GAGCGTTTCCGTTGTCCTGA | Q-PCR |
Actin-R | AGGTGCTGAGGGAAGCCAAA | Q-PCR |