Figure 6. PVR expression on CD4⁺ T cells in peripheral blood and lymph node.

(a) Gating strategy for PVR⁺ cells of total CD4⁺ T cells from a representative HIV+ and healthy control (HC) subject. (b) Frequency of PVR⁺ cells of CD4⁺ T cells in HIV+ (n = 10) and HC (n = 7) subjects in lymph node and peripheral blood. (c) Gating strategy for PVR expression on T follicular helper (Tfh) cells and histogram showing PVR expression of Tfh cells (red) and memory CD4⁺ T cells (blue). (d) Frequency of PVR⁺ cells of Tfh cells versus memory CD4⁺ T cells in lymph nodes of HIV infected subjects (n = 10). (e) Correlation analysis of the frequency of CD38⁺HLA-DR⁺ cells versus frequency of PVR expression on Tfh cells (red) and CD4⁺ memory T cells (blue). (f) Comparison between the frequency of CD38⁺HLA-DR⁺ cells of Tfh versus CD4⁺ memory cells in the lymph nodes from HIV infected subjects (n = 10). The Mann-Whitney test was used for comparisons between two groups. The Wilcoxon matched-pairs signed rank test was used to compare paired samples. The Spearman non-parametric test was used for correlation analysis. **P < 0.01 and ***P < 0.001.