Table I.
Growth of Brassica oleracea as affected by pedospheric and atmospheric sulfur nutrition
Growth Rate
|
Biomass Production
|
||
---|---|---|---|
t6 − t2 | t10 − t6 | t10 − t0 | |
g g−1 day−1 | g | ||
Leaves | |||
−S | 0.201 ± 0.040 | 0.106 ± 0.006* | 2.42 ± 0.46 (63)* |
−S, H2S | 0.205 ± 0.019 | 0.212 ± 0.016 | 3.99 ± 0.72 (104) |
+S | 0.211 ± 0.008 | 0.197 ± 0.009 | 3.84 ± 0.82 (100) |
+S, H2S | 0.203 ± 0.037 | 0.210 ± 0.005 | 4.26 ± 0.95 (111) |
Stem Plus Petioles | |||
−S | 0.279 ± 0.043 | 0.129 ± 0.017* | 0.76 ± 0.15 (65)* |
−S, H2S | 0.257 ± 0.023 | 0.252 ± 0.044 | 1.17 ± 0.17 (100) |
+S | 0.234 ± 0.012 | 0.257 ± 0.011 | 1.17 ± 0.21 (100) |
+S, H2S | 0.282 ± 0.071 | 0.258 ± 0.028 | 1.44 ± 0.30 (123) |
Roots | |||
−S | 0.248 ± 0.072 | 0.128 ± 0.029* | 1.09 ± 0.22 (89) |
−S, H2S | 0.243 ± 0.067 | 0.242 ± 0.026 | 1.76 ± 0.50 (143)* |
+S | 0.186 ± 0.026 | 0.200 ± 0.021 | 1.23 ± 0.41 (100) |
+S, H2S | 0.189 ± 0.048 | 0.195 ± 0.019 | 1.18 ± 0.38 (96) |
B. oleracea was grown on a 25% Hoagland nutrient solution (0.5 mm sulfate) in a climate controlled room for 7 d and subsequently transferred to a fresh nutrient solution at 0 mm sulfate (−S) or 0.5 mm sulfate (+S) and simultaneously exposed to 0 or 75 nL L−1 H2S for 2, 6, and 10 d. Growth rates were measured over the period from day 2 to day 6 (t6 − t2) and from day 6 to day 10 (t10 − t6), and biomass production (fresh weight) was measured over the entire 10 d period (t10 − t0). Growth rate was calculated using the natural log transformed fresh weight. Data represent the mean of 3 independent experiments on 3 to 4 (4–12 for t0) measurements per experiment with 3 plants in each (± sd). Data between brackets represent values as percent of the control (+S). Values marked with an asterisk are significantly different from the control value (+S; P < 0.01).