Figure 2. PMW is CRYPTOCHROME- and GLASS-dependent.

(a) Three-day temperature-shift paradigm to test whether PMW is an acute avoidance response. Ambient temperature is raised from 22 °C to 30 °C at ZT12 on Day 2 for 24 h. Sleep latency was subsequently measured on Day 3 (black arrowhead). (b) Average sleep patterns of control adult male flies during the above temperature-shift paradigm. Subsequent days are juxtaposed to allow direct comparison. Day 1: blue, Day 2: orange, Day 3: red. Black arrowhead indicates PMW during Day 3. n = 43. (c) Comparison of the PMW when ambient temperature is increased at either ZT0 on the experimental day, or at ZT12 – the beginning of the previous night. ns – p > 0.05, Mann-Whitney U-test. ZT0: n = 79 (data also presented in Fig. 1); ZT12: n = 45. (d) PMW in light-pathway mutants. Statistical comparison: Wilcoxon signed rank test compared to a theoretical median of zero. norpA^P41: n = 31, GMR-hid: n = 38, gl^60j: n = 44, cry⁰²: n = 58.