Skip to main content
. 2017 Jan 13;7:40304. doi: 10.1038/srep40304

Figure 4. TrpA1-expressing TrpA1[SH]- and ppk-neurons are necessary for PMW.

Figure 4

(a–c) Average sleep patterns of adult males containing a UAS-TrpA1 RNAi transgene but lacking a promoter-GAL4 driver (a), or expressing UAS-TrpA1 RNAi under control of the TrpA1[SH]- and ppk-GAL4 drivers (b,c respectively). Temperature-shift paradigms are indicated above. Black arrowheads: presence/absence of PMW. (d) Comparison of PMW in males expressing UAS-TrpA1 RNAi under GAL4 drivers and their corresponding controls. n = 32–120. Statistical comparison: Kruskal-Wallis test with Dunn’s post-hoc test. All controls are ***p < 0.0001. [SH] > RNAi: p = 0.0481; ppk > RNAi: p = 0.2420; ppk[200871] > RNAi: p = 0.0054, Wilcoxon signed rank test compared to a theoretical median of zero. (e) Effect of acute inhibition of synaptic output (using UAS-shits) from TrpA1[SH]-, ppk-, and ppk[200871]-neurons on PMW. Statistical comparison: Kruskal-Wallis test with Dunn’s post-hoc test. n = 24–77. All controls except ppk[200871] > + (**p = 0.0008) are ***p < 0.0001. [SH] > shits: ns (p = 0.06); ppk > shits: ns (p = 0.89); ppk[200871] > shits: ***p < 0.0001, Wilcoxon signed rank test compared to a theoretical median of zero. (f) Expression patterns of TrpA1[SH]-, ppk- and ppk[200871]-GAL4 in the adult Drosophila brain. CD4::TdTom is labeled with anti DsRed. Synaptic neuropil (BRP) is labeled using an anti-nc82 antibody. Stars: cell bodies labeled by TrpA1[SH]-GAL4. Arrows: projections to the dorsal posterior protocerebrum from subsets of TrpA1[SH]- and ppk-neurons. Similar projection were not observed in ppk[200871]-positive neurons. Scale bar: 100 μm.