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. 2017 Jan 13;7:40800. doi: 10.1038/srep40800

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) 15-mer overlapping peptide collection covering the HR2 (OLP peptides #155–161) and the MPER (OLP peptides #162–166) used for mapping. (b) OD values of the indicated immunized mice sera (60-fold dilution) against OLP peptides in ELISA. 2F5 binding is also indicated. Color code is shown. (c) For each animal the peptide yielding the strongest signal was assigned 100%. Bar diagram shows the percentage of signal of each peptide in all animals (mean+/− SD), highlighting the immunodominance of the #162 peptide sequence. (d) Spearman’s correlation of #162 binding signals and gp41-Min titer of immunized mice sera. Data are representative of two independent experiments. Spearman’s correlation coefficient and p value are indicated.