Table 1. Gp41-MinTT-containing immunogens used in this study.
| FORMULATION | LIPID COMPOSITION (molar ratio) | PROTEIN | DOSE/INOCULUM |
|---|---|---|---|
| Recombinant protein | gp41-MinTT | 20 μg | |
| Simple proteoliposomes | POPC (100) | gp41-MinTT | 2 μg |
| POPC:GM3 (95:5) | gp41-MinTT | 2 μg | |
| POPC:PS (95:5) | gp41-MinTT | 2 μg | |
| POPC:GM3:PS (90:5:5) | gp41-MinTT | 2 μg | |
| Complex proteoliposomes | POPC:SM:CHOL (30:25:45) | gp41-MinTT | 2 μg |
| POPC:SM:CHOL:GM3 (30:20:45:5) | gp41-MinTT | 2 μg | |
| POPC:SM:CHOL:PS (25:25:45:5) | gp41-MinTT | n.d.* | |
| POPC:SM:CHOL:GM3:PS (25:20:45:5:5) | gp41-MinTT | 2 μg | |
| Complex proteoliposomes + MPLA | POPC:SM:CHOL:MPLA (29:25:45:1) | gp41-MinTT | 2 μg |
| POPC:SM:CHOL:GM3:MPLA (29:20:45:5:1) | gp41-MinTT | 2 μg | |
| POPC:SM:CHOL:PS:MPLA (24:25:45:5:1) | gp41-MinTT | 2 μg | |
| POPC:SM:CHOL:GM3:PS:MPLA (24:20:45:5:5:1) | gp41-MinTT | 2 μg |
Immunogens were categorized in recombinant protein (gp41-MinTT), simple (POPC), complex (POPC CHOL SM) and complex + MPLA (POPC CHOL SM MPLA) proteoliposomes. GM3, PS or both were added to each category. Percentages are referred to lipid components. Lipid mole ratios were used. POPC: 1-Palmitoyl-2-oleoylphosphatidylcholine; CHOL, cholesterol; SM, sphingomyelin; GM3, monosialodihexosylganglioside; PS, phosphatidylserine; MPLA, monophosphoryl lipid A.
*n.d.: not determined.