Figure 3. Reduction in microglial activity in the cortices of Hexb^−/− FcRγ^−/− mice at 4 weeks.

(A) Immunostaining of coronal sections for CD68 (green) and Iba1 (red) in the cerebral cortices of Hexb^+/− FcRγ^+/+, Hexb^−/− FcRγ^+/+, and Hexb^−/− FcRγ^−/− mice at 4 weeks. Blue represents DAPI staining. Insets (a–c) show magnified views of the boxed regions. Scale bar, 100 μm. (B,C) Quantitative analysis for the number of CD68+ (B) and Iba1+ (C) cell immune signals in the cerebral cortices of Hexb^+/− FcRγ^+/+, Hexb^−/− FcRγ^+/+, and Hexb^−/− FcRγ^−/− mice at 4 weeks. Boxes, 25^th–75^th percentile with the median indicated; bars, 10^th and 90^th percentiles. Analyzed using a Kruskal–Wallis test (nonparametric ANOVA) followed by a Dunn’s post hoc test (n = 5). *P < 0.05. N.S.: difference not significant.