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. Author manuscript; available in PMC: 2017 Aug 3.
Published in final edited form as: Neuron. 2016 Jun 30;91(3):574–586. doi: 10.1016/j.neuron.2016.06.021

Figure 5. The extent of muscarinic receptor activation is regulated by the enzymatic degradation of ACh.

Figure 5

(A) Representative sIPSCs recorded under control conditions and in the presence of the AChE inhibitor ambenonium (10 nM).

(B) Averaged superimposed sIPSCs from the above cell under control conditions and in the presence of ambenonium (10 nM) illustrating that inhibiting AChE increases the amplitude and duration of sIPSCs.

(C) Summary of the effect of ambenonium (10 nM) on 10 – 90% rise time and duration (260 ± 24% control width) on sIPSCs (n = 6, p < 0.05, Wilcoxon matched-pairs signed rank).

(D) Amplitude distribution histograms (20 pA bin size) of sIPSCs in control and in the presence of ambenonium (10 nM) (p < 0.001, Kolmogorov-Smirnov test).

(E) Representative trace illustrating the effect of optogenetic silencing of eNpHR-expressing CHIs on muscarinic IPSCs in the presence of ambenonium (80 nM).

(F) Summary of the average baseline current amplitude measured before and after optogenetic silencing of eNpHR-expressing CHIs under control conditions and in the presence of ambenonium (80 nM) (p < 0.05, Mann-Whitney).