Table 1. Summary of the antibodies used during Western blotting to identify the presence of decorin, biglycan, aggrecan, fibromodulin and lumican core proteins and KS glycosaminoglycan chains and CS neo-epitopes (‘stubs’) following enzymatic treatment.

Antibody	Dilution	Detects	Epitope	Reference
mAb DS-1	1:125	Decorin	C-terminal of core protein	[44]
pAb PR-85	1:500	Biglycan	C-terminus (CGG)TDRLAIQFGNYKK	[45]
mAb 6B4	1:50	Aggrecan	Amino acids 413–424 of human aggrecan	[46]
pAb PR-184	1:500	Fibromodulin	C-terminus (CGG)LRLASLIEI	[45]
pAb PR-586	1:500	Lumican	C-terminus (CGG)LRVANEITVN	[47]
mAb 5D4	1:500	KS	Over-sulfated KS heptasaccharides	[48]
mAb 1B4	1:2500	KS	Low-sulfated KS	[49]
mAb 2B6	1:50	C-4-S ‘stubs’	4-sulfated unsaturated disaccharide CS neo-epitopes	[50]
mAb 3B3	1:50	C-6-S ‘stubs’	6-sulfated unsaturated disaccharides CS neo-epitopes	[51]

All antibodies were provided by B. Caterson (Cardiff University) except for those against biglycan, fibromodulin and lumican that were kindly provided by Peter Roughley (McGill University), and to decorin (purchased from Developmental Studies Hybridoma Bank, Iowa).