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. 2017 Jan 13;17:9. doi: 10.1186/s12870-016-0950-x

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Validation of relative expression levels of selected genes (qRT-PCR). Expression levels were compared between I. batatas (Ib; indicated in blue) and I. trifida (It; indicated in red). Quantitative RT-PCR was performed with three biological replicates and two technical replicates for both Ib and It. The sweetpotato β-tubulin gene was used as an endogenous control and the gene expression levels were determined using the ΔΔCt method. Genes are shown with respective TAIR locus IDs: β-amylase (AT4G15210); ADP-glucose pyrophosphorylase (AT4G39210); Starch synthase (AT4G18240); KNOX: class I Knotted-like homeobox (AT4G08150); Expansin (AT2G39700); EPSP: 5- enolpyruvylshikimate-3-phosphate synthase (AT2G45300); ERF: Ethylene-responsive transcription factor (AT1G50640); CoMT: caffeoyl-CoA O-methyltransferase (AT4G34050); CAD: cinnamyl alcohol dehydrogenase (AT1G72680)