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In vivo fluorescent imaging of Cy5 dye in mice. (a) Representative fluorescent image of Cy5 dye in different concentrations [(I) 7.5, (II) 3.75, (III) 1.88, and (IV) 0.94 mM] using the portable imager. Linear relationships between dye concentrations and fluorescent intensities were determined based on imaging results obtained from (b) the portable imager and (c) the Kodak system.
