Fig. 4. (A) All biliary cancer cell line showed apurinic/apyrimidinic endodeoxyribonuclease1 (APEX1) and Jagged1 expression by Western blot. APEX1 and Jagged1 protein expression was detected by western blot analysis with α-tubulin as a loading control. (B) Western blot analysis of APEX1 and Jagged1 expression in biliary cancer cell lines after APEX1 knockdown. Western blot of all biliary cancer cells treated with APEX1 siRNA. Samples were collected 48 hours after APEX1-siRNA treatment, and Western blot was done with APEX1 antibody and reproved with α-tubulin antibody as a loading control.
