Table 3.
Extraction efficiency of MS2 phage-like particles (MS2 PLP) isolated from different types of matrices artificially contaminated with 5 × 106 MS2 PLP per sample.
| Matrix | Number of MS2 phage-like particles isolated from samples1 | Positive samples/analyzed samples | Extraction efficiency | ||
|---|---|---|---|---|---|
| Mean | SD | Mean | SD | ||
| Swab | 2,66 × 106 | 7,74 × 105 | 20/20 | 41,60% | 12,09 |
| Liver tissue | 1,45 × 106 | 7,72 × 105 | 20/20 | 5,28% | 2,63 |
| Serum | 6,10 × 106 | 1,76 × 106 | 20/20 | 77,04% | 22,22 |
| Feces | 7,40 × 105 | 1,80 × 105 | 20/20 | 8,77% | 2,14 |
| Vegetable | 2,99 × 105 | 1,89 × 105 | 18/18 | 4,29% | 2,71 |
1Total amount of MS2 PLP from each sample was determined according to the calibration curve included in each RT-qPCR experiment. RT-qPCR was run in duplicate for each sample. Nucleic acid isolation efficiency was calculated according to the formula described within the text. SD, standard deviation.