Fig. 7.

Repression of VEGF-A protein production by various transcription repression domains in normoxia and hypoxia. The plasmid encoding DRP with various repression domains (0.1 μg) and pCMV-β-galactosidase plasmid (0.01 μg) were cotransfected into 1 × 10⁵ HEK293 cells. Cells were allowed to grow for 12 h and then treated with (hypoxia, open bar) or without (normoxia, filled bar) 150 μM CoCl₂. After 24 h, the culture medium and the transfected cells were collected separately. The VEGF concentration and the β-galactosidase activities were quantified as described in Materials and Methods. Results were normalized for transfection efficiency with β-galactosidase activities. Each point represents the mean ± SD obtained from three independent experiments.