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. 2004 Oct 18;101(43):15416–15421. doi: 10.1073/pnas.0406933101

Fig. 1.

Fig. 1.

Subcellular localization of IRFs and MyD88. (a) Confocal images of HEK293T cells transiently expressing IRF-3YFP, IRF-7YFP, MyD88CFP, or TRIFCFP. (b) Confocal images of HEK293T cells coexpressing MyD88CFP with IRF-3YFP or IRF-7YFP. (c) HeLa cells were transfected with expression plasmids for IRF-7YFP or IRF-3YFP and MyD88CFP. FRET, YFP, and CFP images were collected by using an inverted fluorescence microscope equipped with a cooled charge-coupled device camera. FRETC was determined as described in the text and presented in the pseudocolor mode as indicated below the photographs.