Fig. 10.

Effect of DGAT1 and DGAT2 inhibition on the incorporation of U-¹⁴C glucose into CO₂ and TAG after β3-agonist stimulation of primary brown adipocytes. Cells were incubated with CL for 2 h before the start of incubations by the addition of U-¹⁴C-glucose label. Inhibitors were added individually 30 min before the addition of label (see Methods section). Experiments were performed in the presence of oleate (0.75 mM with 0.25% BSA) and glycerol (0.75 mM). Incorporation of label from U-¹⁴C-glucose was measured for a 2 h period into (A) CO₂, (B) TAG-acyl, and (C) TAG-glyceryl moieties_. The concentrations of inhibitors used were: DGAT1-iB, 0.75 µM; DGAT2-iC, 50 µM, and DGAT2-iJ, 50 µM. Values are means (± SEM) for three separate experiments and are expressed with respect to values for controls (set at 100%) to which no CL or inhibitors were added. Values that were statistical significantly different (P < 0.05) are indicated by * (CL vs. Control) and # (CL + inhibitors vs. CL only). See Methods section for details of statistical analysis. Etmox, etomoxir.