Fig. 3.

Etomoxir selectively prevents the increase in glucose-derived CO₂ formation after β3-adrenergic stimulation of IMBAT-1 cells but not the increased incorporation into the glyceryl and acyl moieties of TAG. Cells were incubated with CL for 2 h, followed by a further incubation period of 2 h, at the start of which U-¹⁴C-glucose label was added (at zero time) as described in the Methods section. Incorporation of label was measured into (A) CO₂ and (B) glyceryl- and (C) acyl-moieties of TAG. Etomoxir was added 30 min before the addition of label. Data are means (± SEM) for three separate experiments and are expressed with respect to paired controls, which are set at 100% for each experiment. Values that were significantly statistically different (P ≤ 0.05) are indicated by * (vs. control) or # (CL+etomoxir vs. CL). See Methods section for details of statistical analyses.