Fig. 4.

ATGL inhibition prevents β3-induced stimulation of U-¹⁴C-glucose incorporation into TAG and CO₂. Cells were incubated with CL for 2 h before addition of U-¹⁴C-glucose, and incorporation of label into (A) CO₂ and (B) TAG was measured during a further 2 h incubation (see Methods section). When Atglistatin (Astat) or THL were present, they were added at the same time as CL. Data are means (± SEM) for three separate experiments and are expressed with respect to control values that were set at 100% for each experiment. Values that were statistically significantly different (P < 0.05) are indicated by * (CL vs. control) and # (CL+THL or CL+ Atglistatin vs. CL).