Fig. 5.

Effect of DGAT1 and DGAT2 inhibition on the incorporation of U-¹⁴C glucose into CO₂ and TAG after β3-agonist stimulation of IMBAT-1 cells. Cells were incubated with CL for 2 h before the start of incubations by the addition of U-¹⁴C-glucose label. Inhibitors were added individually 30 min before the addition of label (see Methods section). Experiments were performed in the absence (A–C) or presence (D–F) of oleate (0.75 mM with 0.25% BSA) and glycerol (0.75 mM). Incorporation of label from U-¹⁴C-glucose was measured for a 2 h period into CO₂ (A, D), TAG-acyl moieties (B, E), and TAG-glyceryl moieties (C, F). The concentrations of inhibitors used were: DGAT1-iB, 0.75 µM; DGAT2-iC, 50 µM, and DGAT2-iJ, 50 µM. Values are means (± SEM) for three separate experiments and are expressed with respect to values for controls (set at 100%) to which no CL or inhibitors were added. Values that were statistical significantly different (P < 0.05) are indicated by * (CL vs. control) and # (CL + inhibitors vs. CL only).