Fig. 7.

Effects of siRNA-mediated DGAT1- or DGAT2-knockdown on the rates of incorporation of U-¹⁴C-glucose into CO₂ and TAG in the presence or absence of β3-adrenergic stimulation of IMBAT-1 cells. Differentiated cells were treated for 72 h with either control (scrambled, SC) siRNA or siRNA (SiD) targeted toward DGAT1 (SiD1) or DGAT2 (SiD2) (see Methods section). Cells were incubated +/− CL for 2 h followed by the measurement of the incorporation of label from U-¹⁴C-glucose into (A) CO₂, (B) total TAG, and (C, E) TAG-glyceryl and (D, F) TAG-acyl moieties of TAG. Data are means (± SEM) for three separate experiments and are expressed with respect to Control values (which are set at 100%) for each experiment. Values that are statistically significantly different (P < 0.05) are indicated by * (SC+CL or SiD vs. SC) and # (SiD+CL vs. SC+CL).