Fig. 8.

Effect of siRNA-mediated knockdown of DGAT1 and DGAT2 on the expression of genes involved in pathways leading from glucose to TAG-synthesis and CO₂ formation. Differentiated cells were treated for 72 h with control siRNA (scrambled, SC) or siRNA targeted against DGAT1 (SiD1) or DGAT2 (SiD2). mRNA determinations were performed on cells incubated for 2 h either in the absence (-) or presence (+) of CL. The variances of input cDNA were normalized against the levels of three housekeeping genes: L19, B-actin, and 36B4, and expressed relative to those of SC, which were set at 1.0. Values are means (± SEM) for three separate experiments. Values that were statistically significantly different (P < 0.05) are indicated by * (vs. SC) and # (vs. SC+CL). ACC1, acetyl-CoA carboxylase 1; FASN, fatty acid synthase.