Fig. 3.

Exogenous alkyne-doxSA localizes to mitochondria. Subcellular tracking of alkyne-doxSA by fluorescence microscopy. MEF cells were treated with a nontoxic concentration of 0.1 μM alkyne-doxSA for 5 min (A), 1 h (B), or 24 h (C). After treatment for the indicated times, cells were fixed and immunostained for organelle protein markers (magenta), the alkyne moiety was reacted with ASTM-BODIPY (green), and the cells analyzed by fluorescence microscopy. Scale bars, 10 μm. D: Analysis of the metabolic fate of the alkyne probe by click-fluorescence TLC. BG, background.