Fig. 4.

Ox-HDL induces intracellular lipid accumulation in RAW264.7 cells. A: Dil-ox-HDL and Dil-ox-LDL fluorescence intensity in cells incubated with Dil-ox-HDL and Dil-ox-LDL at the indicated concentrations for 6 h. Scale bar = 20 μm. B, C: Cells were treated with the indicated concentration of ox-HDL, n-HDL (100 mg/l), or ox-LDL (100 mg/l) for 24 h, and the levels of intracellular lipid were determined using oil red O staining and Nile red staining, respectively. Representative lipid droplet staining images are shown. Scale bar = 20 μm. Data are expressed as the mean ± SD of at least four independent experiments. *P < 0.05; **P < 0.01 versus control group; ^##P < 0.01.