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. 2016 Dec 29;58(1):60–71. doi: 10.1194/jlr.M072421

Fig. 6.

Fig. 6.

Levels of d3-deoxySA-OH and d3-deoxySA-2OH significantly increase upon overexpression of Cyp4f13. HEK WT cells or HEK cells overexpressing either mouse Cyp4a10 or Cyp4f`13 were treated with d3-deoxySA for 48 h, and the d3-labeled 1-deoxySL downstream metabolites were measured by LC/MS. Levels of d3-deoxySA-OH (A) and d3-deoxySA-2OH (B) were significantly higher in Cyp4f13-overexpressing cells. Levels of d3-deoxySO-OH (C) did not differ between the three cell lines. The sum of all four d3-deoxySA-2OH peaks and both d3-deoxySO-OH peaks is shown. D: Western blot of CYP4F13 and CYP4A10 expression. Bars represent averages ± SEM. Statistical significance was calculated using one-way ANOVA followed by Bonferroni correction. **P < 0.01, ***P < 0.001; n.s., not significant.