Fig. 1.

Western blots with GPIHBP1-specific monoclonal antibodies. Soluble versions of wild-type (wt) human GPIHBP1, GPIHBP1-W109S, and GPIHBP1-Δacidic (in which GPIHBP1’s acidic domain had been deleted) were expressed in Drosophila S2 cells; all constructs had a carboxyl-terminal mAb 11A12 epitope tag (9). Western blot analysis was performed on the conditioned medium of the Drosophila S2 cell cultures under nonreducing conditions using IRDye800-labeled GPIHBP1-specific mAbs RG3, RE3, RF4, RH1, and RE6 (green) and an IRDye680-labeled mAb 11A12 (red). Monomers are indicated by arrowheads.