Table 3. Analysis of the effect of pH on TP3 and TP4 antimicrobial activity.
| ampicillin | kanamycin | TP3 | TP4 | |||||
|---|---|---|---|---|---|---|---|---|
| MIC (μg/ml) | MBC (μg/ml) | MIC (μg/ml) | MBC (μg/ml) | MIC (μg/ml) | MBC (μg/ml) | MIC (μg/ml) | MBC (μg/ml) | |
| pH = 2 | ND | ND | ND | ND | ND | ND | ND | ND |
| pH = 4 | 3.9 | 3.9 | 31.2 | 31.2 | 125 | 125 | 15.6 | 15.6 |
| pH = 6 | 3.9 | 3.9 | 31.2 | 31.2 | 62.5 | 62.5 | 7.8 | 7.8 |
| pH = 8 | 3.9 | 3.9 | 31.2 | 31.2 | 62.5 | 62.5 | 7.8 | 7.8 |
| pH = 10 | 3.9 | 3.9 | 31.2 | 31.2 | 125 | 125 | 15.6 | 15.6 |
| pH = 12 | 15.6 | 15.6 | 250 | 250 | 250 | 250 | 31.2 | 31.2 |
| Control | 3.9 | 3.9 | 31.2 | 31.2 | 62.5 | 62.5 | 7.8 | 7.8 |
Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ampicillin, kanamycin, TP3, and TP4 on V. vulnificus. Antibiotics and peptides were dissolved in different pH solutions.