Figure 3. In vivo LDA identifies low-affinity CD4+ T-cell clones.

(a) Representative NP_311–325 Tet staining in TCRα^−/− LDA mice with both a Tet+ positive (left) and negative (right), with only 1 out of 34 mice possessing a Tet+-positive clone. (b) MP on single CD4+ T cells isolated from positive and negative LDA mice as identified by CD69/Nur77 (data combined from two independent experiments, n=2–3 mice, Student's t-test, *P=0.0073). MP affinities on individual positive LDA clones following NP_311–325/CFA immunization along with a separate analysis of the polyclonal repertoire (single points represent individual cells). (c) Single-cell TCRβ sequencing from LDA-positive and -control mice. Individual colours represent unique TCRβ CDR3 sequences, with each column representing a single mouse.