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. 2017 Jan 13;12(1):e0170441. doi: 10.1371/journal.pone.0170441

Fig 5. RNA expression and promoter methylation changes in genes coding for RNA subtypes after prenatal exposure to DEHP.

Fig 5

(A, B, C) Analysis of transcript levels and promoter methylation across the RNA subtypes. Orange dots and bars represent sixty-seven detected C/D box Snords. Blue dots and bars represent nine seminal vesicle secretory genes (Sva, Svs1, Svs2, Svs3a, Svs3b, Svs4, Svs5, Svs6, and Pate4). Black dots and bars represent nine hundred and eighty-six microRNAs. Red dots and bars represent nine upregulated genes in the sperm of DEHP exposed mice (Rbp2, mir615, C1qtnf5, Gzmk, Gm8994, Gm6329, Fbxw15, 231002L09Rik, and 1700044K03Rik). (A) Scatterplot of transcript levels in FPKM with the CTL group on the vertical axis and the D300 group on the horizontal axis. (B) Boxplot of FPKM expression across RNA subtypes and under experimental conditions. (C) Boxplot of methylation in MBD-seq reads per CpG in 2.2 kb probed promoter regions across RNA subtypes and conditions. Stars represent significant differences between CTL and D300 groups (results are shown in Table 2). (D) Reads mapped to the cluster of Svs genes in chromosome 2 across the 10 samples. Five CTL samples up and five D300 down from the horizontal dashed line. Reads in blue and red differ according to their orientation; genes orientations are shown with arrows.