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. 2017 Jan 3;13(1):e1006116. doi: 10.1371/journal.ppat.1006116

Fig 5. The B. dolosa fixLJ deletion mutant is less motile.

Fig 5

(A) B. dolosa strain AU0158, its fixLJ deletion mutant, and the fixLJ deletion mutant complemented with fixLJ under the control of its own promoter or empty vector (EV) were plated on low-density (0.3%) LB agar and swimming distance was measured after incubation for 48 hours. *P<0.05 by 1-way ANOVA with Tukey’s multiple comparison test. (B) The B. dolosa fixLJ -deletion mutant complemented with fixLJ under the control of a rhamnose-inducible promoter or empty vector grown in the presence of glucose (0.4%, which represses fixLJ expression) or rhamnose (0.4%). *P<0.05 by 1-way ANOVA with Tukey’s multiple comparison test relative to the complemented strain (ΔfixLJ + fixLJ) grown in rhamnose-containing media. For all panels, bars represent mean measurements of 3–4 replicates and error bars represent one standard deviation (representative of three independent experiments).