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. 2017 Jan 13;12(1):e0169537. doi: 10.1371/journal.pone.0169537

Fig 1. Collaborative enhancement binding of anti-muPECAM-1 [125I]-mAb Mec13.3 in live cells.

Fig 1

Binding curves of [125I]-mAb Mec13.3 alone (“solo”) or in the presence of unlabeled mAb 390 to paired epitope (“paired”) in live cells stably expressing recombinant muPECAM-1 (RmP) (A) and mutant form of PECAM-1 (RmPK89A) (B) determined by RIA-based method. Increasing concentrations of [125I]-mAb treatments solo or with paired mAb were added to cells and incubated at 4°C for 2h. The results are presented as total binding corrected for nonspecific binding on REN-WT cells. Decrease in Kd corresponds to the increase in binding affinity. In RmP cells, Kd of Mec13.3 sees 0.48 ± 0.03-fold change in solo vs paired, P<0.001. In RmPK89A cells, Kd changed 0.06 ± 0.05-fold, P<0.001. The insets show Scatchard analysis of experiments.