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. Author manuscript; available in PMC: 2018 Jan 10.
Published in final edited form as: Cell Rep. 2017 Jan 10;18(2):545–556. doi: 10.1016/j.celrep.2016.12.034

Figure 4. The regulation of smad2 pre-mRNA splicing during gastrulation by Rpl22 and Like1 is associated with their retention in the nucleus.

Figure 4

(A,B) Subcellular location of epitope-tagged Rpl22 and Like1 at 10hpf (A) and 24hpf (B). 100pg of mRNA encoding HA-zRpl22 (HA/L22) and HA-zLike1 (HA/L1) was co-injected with mRNA encoding mCherry-CAAX into 1-cell stage embryos and visualized by HA antibody immunostaining. mCherry-CAAX marked the cell membrane and DAPI marked the nucleus. Red scale bar =10 µm. (C) RT-PCR detection of smad2 mis-splicing. Following Like1 MO injection, smad2 mis-splicing was assessed by RT-PCR in 10hpf and 24hpf Like1 morphants. All results are representative of at least 3 experiments performed. See also Figure S4.

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