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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Neurobiol Aging. 2016 May 21;45:88–97. doi: 10.1016/j.neurobiolaging.2016.05.015

Figure 1. Aging reduces tonic activation of presynaptic GABAB autoreceptors on inhibitory inputs to layer 2/3 pyramidal neurons.

Figure 1

A) Raw data traces representative of the population mean are illustrated from a young neuron (top) and an aged neuron (bottom) in baseline conditions, after application of 10 μM baclofen, and in the presence of both baclofen and 10 μM CGP. Each trace is an average of 15–30 consecutive sweeps (generated at 0.1 Hz) in the stated conditions. Note that while baclofen reduced evoked IPSC amplitude in both cells, amplitude in the presence of baclofen and CGP exceeded baseline amplitude much more strongly in the neuron extracted from a young animal. The top dashed line in each set of traces illustrates a common baseline, while the bottom dashed line represents the evoked IPSC amplitude in baseline conditions. B) Population data illustrate that the same effects are apparent across cells when evoked IPSC amplitude is normalized to the baseline mean (young: n=39, 8, aged: n=23, 7, in neurons, animals respectively). See text for specific values. DNQX (10 μM) and APV (40 μM) were present in the bath solution throughout the experiment.