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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Neurobiol Aging. 2016 May 21;45:88–97. doi: 10.1016/j.neurobiolaging.2016.05.015

Table 2. Aging does not alter frequency or amplitude of spontaneous glutamatergic or GABAergic inputs to L2/3 pyramidal neurons.

Values presented in this table were obtained from L2/3 pyramidal cells voltage clamped at −70 mV. Spontaneous IPSCs were isolated using a high chloride CsMeSO3 based internal solution and bath antagonists for ionotropic glutamate receptors (see Methods, Results). Spontaneous EPSCs were isolated using a lower chloride CsMeSO3 based internal solution and a bath antagonist for GABAA receptors. See Methods, Results for more details. In all experiments, basal spontaneous event frequency and amplitude were quantified over a 5 min period that began approximately 15 minutes after initiation of whole cell recording. Cells without stable event frequency during that time, or with unstable access resistance or holding current, were discarded from the dataset. N values represent neurons and animals, respectively.

Frequency (Hz) Amplitude (pA) N values
Young Aged p Young Aged p Young Aged
Isolated sIPSCs 2.0 ± 0.26 1.7 ± 0.43 0.53 17.5 ± 0.92 16.2 ± 0.76 0.29 37, 15 31, 13
Isolated sEPSCs 7.1 ± 0.96 5.1 ± 0.95 0.17 12.9 ± 1.11 12.3 ± 0.75 0.68 13, 5 11, 4