Fig. 1.

CVA induces differentiation to the erythroid lineage. Differentiation was assayed as a measure of the emergence of hemoglobinized cells positive for benzidine. Analysis was carried out with K562, transgenic mice bone marrow progenitor stem cells and JK-1 cells. A. K562 cells induced with CVA for 72 h and stained with Benzidine and Giemsa stains. B. K562 cells were induced with varying concentrations of CVA and monitored for percentage of benzidine positive cells at 48 and 120 h post induction. C. Transgenic mice bone marrow progenitor stem cells depleted of plastic adherent cells in IMDM supplemented with 20% FBS, 100 U/ml penicillin, 200 μg/ml streptomycin and 2 U/ml EPO. Cells were induced with CVA for 96 h, and cytospin preparations of the cells were stained using benzidine-giemsa stain. D. Percentage of BFUe colony formation from TMbmEPSCs induced by CVA. TMbmEPSCs depleted of plastic adherent cells were seeded in plates containing semisolid IMDM supplemented with 20% FBS, 100 U/ml penicillin and 200 μg/ml streptomycin. E. JK-1 cells induced with 50 μM CVA and monitored for percentage of benzidine positive cells at 24 and 72 h post induction.