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. Author manuscript; available in PMC: 2017 Jan 13.
Published in final edited form as: Eur J Pharmacol. 2016 Feb 12;776:9–18. doi: 10.1016/j.ejphar.2016.02.041

Fig. 4.

Fig. 4

CVA lowers KLF1 gene expression in JK-1 cells. JK-1 cells were grown in RPMI 1640 medium supplemented with 20% FBS, 100U/ml penicillin and 200 μg/ml streptomycin, cells induced with CVA (50 μM). KLF1 gene expression was monitored quantitatively using qRT-PCR 24 h post induction. KLF1 gene expression was normalized to JK-1 GAPDH gene expression and compared to un-induced controls. The data represents the mean and corresponding S.D. of three independent experiments. ANOVA was used to analyse the differences between groups and differences were considered significant at P<0.05.