Fig. 5.

Effects of Erythropoietin on CVA induced JK-1 cell differentiation and γ-globin gene expression. EPO has been shown to direct erythroid cell development and maturation (Orkin and Zon, 2008). JK-1 cells were induced with EPO (2 U/ml) for 48 h prior to induction with CVA; cells were then induced using 50 μM CVA, EPO (2 U/ml) and ‘CVA (50 μM)+EPO (2 U/ml)’ for 48 h. Controls were set-up for each treatment using JK-1 cells which have not been induced with EPO. JK-1 cell differentiation and γ-globin gene expression were assayed 48 h post CVA induction. A. Experimental set-up B. JK-1 relative γ-globin gene expression normalized to GAPDH C. JK-1 cell differentiation assayed by benzidine-giemsa staining. The data represents the mean and corresponding S.D. of three independent experiments. ANOVA was used to analyse the differences between groups and differences were considered significant at P<0.05.