Table 1. Primer sequence use for brain mRNA Analysis.
Genes | sequence | |
---|---|---|
POMC | Forward 5’ | CTCCTGCTTCAGACCTCCATAGAT |
Reverse 5’ | GGATGCAAGCCAGCAGGTT | |
CART | Forward 5’ | CCGAGCCCTGGACATCTACTC |
Reverse 5’ | AAATACTGACCAGCTCCTTCTCATG | |
AgRP | Forward 5’ | GTTCCCAGAGTTCCCAGGTCTAA |
Reverse 5’ | TTCTGCTCGGTCTGCAGTTG | |
NPY | Forward 5’ | CTCTGCGACACTACATCAATCTCA |
Reverse 5’ | GTGTCTCAGGGCTGGATCTCTT | |
CRH | Forward 5’ | CAACCTCAGCCGGTTCTGA |
Reverse 5’ | CCCCAGGCGGAGGAAGTA | |
MC4R | Forward 5’ | TAGCCTGGCTGTGGCAGAT |
Reverse 5’ | CGATGGTTTCCGACCCATT | |
Y2R | Forward 5’ | CCGCTCCTGCTTCTGATCTC |
Reverse 5’ | ACCCAAAGCAGGTCCGATT | |
Y5R | Forward 5’ | AACCTTTGGCTCAGCATTGC |
Reverse 5’ | CAGAGGGCCATGACTCAACA | |
OX1R | Forward 5’ | CCGTCTACGCCTGCTTCAC |
Reverse 5’ | AGGGTTGGCGGCACTGT | |
OX2R | Forward 5’ | TGCAAAGACCAGAAGTACAACCA |
Reverse 5’ | CAGATCCGAGCACGAAGGAA | |
HRCT | Forward 5’ | TGGACCACTGCACTGAAGAGA |
Reverse 5’ | CAGGGAACCTTTGTAGAAGGAAAG | |
18S | Forward 5’ | GGGAGCCTGAGAAACGGC |
Reverse 5’ | GGGTCGGGAGTGGGTAATTT | |
RPL13A | Forward 5’ | GGATCCCTCCACCCTATGACA |
Reverse 5’ | CTGGTACTTCCACCCGACCTC |