. 2017 Jan 13;12(1):e0169908. doi: 10.1371/journal.pone.0169908

Table 1. Primer sequence use for brain mRNA Analysis.

Genes		sequence
POMC	Forward 5’	`CTCCTGCTTCAGACCTCCATAGAT`
	Reverse 5’	`GGATGCAAGCCAGCAGGTT`
CART	Forward 5’	`CCGAGCCCTGGACATCTACTC`
	Reverse 5’	`AAATACTGACCAGCTCCTTCTCATG`
AgRP	Forward 5’	`GTTCCCAGAGTTCCCAGGTCTAA`
	Reverse 5’	`TTCTGCTCGGTCTGCAGTTG`
NPY	Forward 5’	`CTCTGCGACACTACATCAATCTCA`
	Reverse 5’	`GTGTCTCAGGGCTGGATCTCTT`
CRH	Forward 5’	`CAACCTCAGCCGGTTCTGA`
	Reverse 5’	`CCCCAGGCGGAGGAAGTA`
MC₄R	Forward 5’	`TAGCCTGGCTGTGGCAGAT`
	Reverse 5’	`CGATGGTTTCCGACCCATT`
Y₂R	Forward 5’	`CCGCTCCTGCTTCTGATCTC`
	Reverse 5’	`ACCCAAAGCAGGTCCGATT`
Y₅R	Forward 5’	`AACCTTTGGCTCAGCATTGC`
	Reverse 5’	`CAGAGGGCCATGACTCAACA`
OX₁R	Forward 5’	`CCGTCTACGCCTGCTTCAC`
	Reverse 5’	`AGGGTTGGCGGCACTGT`
OX₂R	Forward 5’	`TGCAAAGACCAGAAGTACAACCA`
	Reverse 5’	`CAGATCCGAGCACGAAGGAA`
HRCT	Forward 5’	`TGGACCACTGCACTGAAGAGA`
	Reverse 5’	`CAGGGAACCTTTGTAGAAGGAAAG`
18S	Forward 5’	`GGGAGCCTGAGAAACGGC`
	Reverse 5’	`GGGTCGGGAGTGGGTAATTT`
RPL13A	Forward 5’	`GGATCCCTCCACCCTATGACA`
	Reverse 5’	`CTGGTACTTCCACCCGACCTC`