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. 2004 Oct;17(4):903–925. doi: 10.1128/CMR.17.4.903-925.2004

FIG. 3.

FIG. 3.

SYBR Green-based quantitative PCR. SYBR Green-based quantitative PCR utilizes a sensitive DNA binding dye that binds any double-stranded product generated by the PCR. Thus, the same dye can be used in different quantitative PCR approaches, but the specificity must be verified by other methods (see the text).