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Quantitative real-time PCR with melting curve analysis. (A) Detection of HSV DNA in a serially diluted suspension by LightCycler PCR with the FRET assay. The sequential numbers indicated at the base of each signal designation refer to the corresponding sample number and dilution (two left-hand columns). (B) Melting curve analysis of HSV-1 and HSV-2 genotypes determined by LightCycler PCR with the FRET assay. Reprinted from reference 79 with permission.
