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. 2016 Sep 6;101(2):439–448. doi: 10.1189/jlb.2A0316-156RR

Figure 7. Overexpression of ASAP2 with a C86→A mutation leads to lower phagocytic capacity through the FcγR.

Figure 7.

RAW264.7 cells were transfected with a plasmid encoding RFP-ASAP2 or RFP-ASAP2C86→A. The latter plasmid encoded for a version of ASAP2 with the palmitoylation site mutated from a Cys to Ala. Transfected cells were plated onto cover slips, and green fluorescent, IgG-coated microspheres (1 μm) were added to the cells for 30 min; then, the cells were washed and fixed and analyzed for phagocytosed beads by fluorescent microscopy. (A) Representative images show higher phagocytic capacity in the RAW264.7 cells rescued with WT ASAP2 compared to those rescued with the C86→A mutant ASAP2. Scale bar, 10 μm. (B) Phagocytic index (number of beads per cell multiplied by the percentage of cells with ≥1 bead) was calculated for >100 cells/group. Results are expressed as means ± sem. *P < 0.05.